• Users Online: 69
  • Home
  • Print this page
  • Email this page
Home About us Editorial board Ahead of print Current issue Search Archives Submit article Instructions Subscribe Contacts Login 
ORIGINAL ARTICLE
Year : 2013  |  Volume : 2  |  Issue : 2  |  Page : 46-48

Efficacy of Various Stains to Study Mitotic Figures in Oral Epithelial Dysplasia - A Pilot Study


1 Professor & Head, Department of Oral and Maxillofacial Pathology, Sinhgad Dental College and Hospital, Pune, Maharashtra, India
2 P.G. Students, Department of Oral and Maxillofacial Pathology, Sinhgad Dental College and Hospital, Pune, Maharashtra, India
3 Lecturer, Department of Oral and Maxillofacial Pathology, Sinhgad Dental College and Hospital, Pune, Maharashtra, India
4 Reader, Department of Oral and Maxillofacial Pathology, Sinhgad Dental College and Hospital, Pune, Maharashtra, India

Correspondence Address:
Login to access the Email id

Source of Support: None, Conflict of Interest: None


DOI: 10.4103/2277-4696.159281

Rights and Permissions

Background: Although Haematoxylin and Eosin is a widely used stain to study oral epithelial dysplasia, sometimes studying mitotic figures in sections stained with these stains might pose problems. Mitotic figures is one of the major criterias to assess dysplasia. Various methods to illustrate mitotic figures have been developed over the years including microscopy, morphometry, flow cytometry, nucleotide radiolabelling and immunohistochemistry. But these methods are not cost effective and less feasible for routine use. Selective stains such as crystal violet, giemsa, toluidine blue, feulgen have been used for staining mitotic figures in tissues. Aim: The present study aims to evaluate effectiveness of various stains for studying mitotic figures. Objectives: To study the efficacy of various specific stains to highlight mitotic figures in oral epithelial dysplasia. Material & methods: The study sample includes sections from tissues embedded in paraffin blocks diagnosed as oral epithelial dysplasia. These sections will be stained with various stains and the mitotic figures will be assessed. Results: Mitotic figures were enhanced with feulgen stain. Conclusion: The study will be further continued with greater sample size for quantification of mitotic figures with H & E and Feulgen stain.


[PDF]*
Print this article     Email this article
 Next article
 Previous article
 Table of Contents

 Similar in PUBMED
   Search Pubmed for
   Search in Google Scholar for
 Related articles
 Citation Manager
 Access Statistics
 Reader Comments
 Email Alert *
 Add to My List *
 * Requires registration (Free)
 

 Article Access Statistics
    Viewed1273    
    Printed20    
    Emailed0    
    PDF Downloaded1775    
    Comments [Add]    
    Cited by others 1    

Recommend this journal